<?xml version="1.0" encoding="UTF-8"?> <!DOCTYPE book PUBLIC "-//NLM//DTD BITS Book Interchange DTD v2.3 20210610//EN" "BITS-book2.3.dtd"> <book xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:mml="http://www.w3.org/1998/Math/MathML" book-type="conference-proceedings" dtd-version="2.3" xml:lang="ru"> <front> <book-meta>  <book-id book-id-type="isbn">978-5-907965-64-5</book-id>   <book-id pub-id-type="doi">10.31483/a-10716</book-id>   <title-group>  <book-title xml:lang="ru">Фундаментальные и прикладные исследования по приоритетным направлениям биоэкологии и биотехнологии</book-title>   <trans-title-group xml:lang="en"> <trans-title>Fundamental and applied research for key propriety areas of bioecology and biotechnology</trans-title> </trans-title-group>  </title-group>  <description xml:lang="ru"> <p>В сборнике представлены статьи участников VIII Всероссийской научно-практической конференции «Фундаментальные и прикладные исследования по приоритетным направлениям биоэкологии и биотехнологии». В материалах сборника приведены результаты теоретических и прикладных изысканий представителей научного и образовательного сообщества в области биологии и медицины. Статьи представлены в авторской редакции.</p> </description>   <contrib-group>  <contrib contrib-type="editor" id="editor1">  <contrib-id contrib-id-type="role">chief_editor</contrib-id>    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Антонова</surname> <given-names>Елена Ивановна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Antonova</surname> <given-names>Elena Ivanovna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3686-9686</contrib-id> <email xlink:type="simple">antonov_67@mail.ru</email> </contrib>  <contrib contrib-type="editor" id="editor2">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Ленгесова</surname> <given-names>Наталья Анатольевна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Lengesova</surname> <given-names>Natal&#039;ia Anatol&#039;evna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7512-6386</contrib-id> <email xlink:type="simple">lengesova@yandex.ru</email> </contrib>  <contrib contrib-type="editor" id="editor3">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Фирсова</surname> <given-names>Наталья Викторовна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Firsova</surname> <given-names>Natalia Viktorovna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9907-8857</contrib-id> <email xlink:type="simple">n-firsova@mail.ru</email> </contrib>  </contrib-group>   <contrib-group>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm1">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Антонова</surname> <given-names>Елена Ивановна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Antonova</surname> <given-names>Elena Ivanovna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3686-9686</contrib-id> </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm2">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Антонова</surname> <given-names>Елена Ивановна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Antonova</surname> <given-names>Elena Ivanovna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3686-9686</contrib-id> </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm3">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Ленгесова</surname> <given-names>Наталья Анатольевна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Lengesova</surname> <given-names>Natal&#039;ia Anatol&#039;evna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7512-6386</contrib-id> </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm4">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Беззубенкова</surname> <given-names>Ольга Евгеньевна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Bezzubenkova</surname> <given-names>Ol&#039;ga Evgen&#039;evna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3243-6381</contrib-id> </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm5">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Коняев</surname> <given-names>Игорь Сергеевич</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Koniaev</surname> <given-names>Igor&#039; Sergeevich</given-names> </name>  </name-alternatives>   </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm6">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Масленников</surname> <given-names>Андрей Викторович</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Maslennikov</surname> <given-names>Andrei Viktorovich</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1421-7484</contrib-id> </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm7">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Куницына</surname> <given-names>Анастасия Владимировна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Khambikova</surname> <given-names>Anastasiia Vladimirovna</given-names> </name>  </name-alternatives>   </contrib>  <contrib contrib-type="member-of-organizing-committee" id="orgcomm8">    <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Фирсова</surname> <given-names>Наталья Викторовна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Firsova</surname> <given-names>Natalia Viktorovna</given-names> </name>  </name-alternatives>  <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9907-8857</contrib-id> </contrib>  </contrib-group>   <event>  <event-desc xml:lang="ru">Фундаментальные и прикладные исследования по приоритетным направлениям биоэкологии и биотехнологии</event-desc>   <event-desc xml:lang="en">Fundamental and applied research for key propriety areas of bioecology and biotechnology</event-desc>   <conf-date> <day>22</day> <month>05</month> <year>2025</year> </conf-date>    <conf-loc xml:lang="ru">Ульяновск</conf-loc>  </event>   <publisher> <publisher-name>ИД «Среда»</publisher-name> </publisher>    <pub-date date-type="collection" publication-format="electronic" iso-8601-date="1900"> <year>1900</year> </pub-date>    <permissions>  <copyright-statement xml:lang="en">© 2025 Natalia V. Firsova, Tatiana N. Kazantseva, Natal&#039;ia A. Lengesova, Elena I. Antonova</copyright-statement>   <copyright-statement xml:lang="ru">© 2025 Фирсова Н. В., Казанцева Т. Н., Ленгесова Н. А., Антонова Е. И.</copyright-statement>   <copyright-year>2025</copyright-year>  <copyright-holder xml:lang="ru">Фирсова Н. В., Казанцева Т. Н., Ленгесова Н. А., Антонова Е. И.</copyright-holder>   <copyright-holder xml:lang="en">Natalia V. Firsova, Tatiana N. Kazantseva, Natal&#039;ia A. Lengesova, Elena I. Antonova</copyright-holder>    <license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/" xml:lang="en" xlink:type="simple"> <license-p>This work is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0)</license-p> </license>   <license license-type="open-access" xlink:href="https://creativecommons.org/licenses/by/4.0/" xml:lang="ru" xlink:type="simple"> <license-p>Это произведение доступно по лицензии Creative Commons Attribution 4.0 International (CC BY 4.0)</license-p> </license>   </permissions>  </book-meta> <book-part book-part-type="conference-paper"> <book-part-meta>  <book-id pub-id-type="doi">10.31483/r-138740</book-id>   <book-id custom-type="publisher-id" pub-id-type="custom">138740</book-id> <title-group>  <chapter-title xml:lang="ru">Влияние DMSO-криопротектора на биологию фибробластов кожи человека в культуре in vitro</chapter-title>   <trans-title-group xml:lang="en"> <trans-title>The effect of DMSO cryoprotector on the biology of human skin fibroblasts in in vitro culture</trans-title> </trans-title-group>  </title-group>  <contrib-group>   <contrib contrib-type="author" id="author1"> <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9907-8857</contrib-id>  <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Фирсова</surname> <given-names>Наталья Викторовна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Firsova</surname> <given-names>Natalia Viktorovna</given-names> </name>   </name-alternatives>  <email xlink:type="simple">n-firsova@mail.ru</email> <xref ref-type="aff" rid="aff1"/> </contrib>   <contrib contrib-type="author" id="author2">   <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Казанцева</surname> <given-names>Татьяна Николаевна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Kazantseva</surname> <given-names>Tatiana Nikolaevna</given-names> </name>   </name-alternatives>  <email xlink:type="simple">tatian.ca@yandex.ru</email> <xref ref-type="aff" rid="aff2"/> </contrib>   <contrib contrib-type="author" id="author3"> <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-7512-6386</contrib-id>  <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Ленгесова</surname> <given-names>Наталья Анатольевна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Lengesova</surname> <given-names>Natal&#039;ia Anatol&#039;evna</given-names> </name>   </name-alternatives>  <email xlink:type="simple">lengesova@yandex.ru</email> <xref ref-type="aff" rid="aff1"/> </contrib>   <contrib contrib-type="author" id="author4"> <contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-3686-9686</contrib-id>  <name-alternatives>  <name name-style="eastern" xml:lang="ru"> <surname>Антонова</surname> <given-names>Елена Ивановна</given-names> </name>   <name name-style="western" xml:lang="en"> <surname>Antonova</surname> <given-names>Elena Ivanovna</given-names> </name>   </name-alternatives>  <email xlink:type="simple">antonov_67@mail.ru</email> <xref ref-type="aff" rid="aff1"/> </contrib>    <aff-alternatives id="aff1">   <aff xml:lang="ru">  <institution>Научно-исследовательский центр фундаментальных и прикладных проблем биоэкологии и биотехнологии ФГБОУ ВО «Ульяновский государственный педагогический университет им. И.Н. Ульянова»</institution>   <country>Россия</country> </aff>    <aff xml:lang="en">  <institution>Nauchno-issledovatel&#039;skii tsentr fundamental&#039;nykh i prikladnykh problem bioekologii i biotekhnologii FGBOU VO &quot;Ul&#039;ianovskii gosudarstvennyi pedagogicheskii universitet im. I.N. Ul&#039;ianova&quot;</institution>   <country>Russia</country> </aff>   </aff-alternatives>  <aff-alternatives id="aff2">   <aff xml:lang="ru">  <institution-wrap> <institution-id institution-id-type="ror">00j0x9b96</institution-id> <institution>Ульяновский государственный педагогический университет им. И.Н. Ульянова</institution> </institution-wrap>   <country>Россия</country> </aff>    <aff xml:lang="en">  <institution-wrap> <institution-id institution-id-type="ror">00j0x9b96</institution-id> <institution>Ulyanovsk State Pedagogical University named after I. N. Ulyanov</institution> </institution-wrap>   <country>Russia</country> </aff>     </aff-alternatives>  </contrib-group>   <fpage>132</fpage> <lpage>135</lpage>   <abstract xml:lang="ru"> <p>в данном исследовании проведен анализ влияния различных периодов DMSO-криоконсервации на морфофункциональные характеристики дермальных фибробластов.</p> </abstract>           <kwd-group xml:lang="ru">  <kwd>клеточные технологии</kwd>  <kwd>фибробласты</kwd>  <kwd>DMSO</kwd>  <kwd>криоконсервация</kwd>  <kwd>размораживание</kwd>  </kwd-group>        </book-part-meta> </book-part> </front>  <back> <ref-list> <title>References</title>  <ref id="ref1"> <label>1</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Гринчук Т.М. Влияние криоконсервации на стабильность кариотипа трансформированных фибробластов легкого китайского хомячка in vitro / Т.М. Гринчук, М.А. Шилина // Цитология. – 2021. – T. 63. №1. – С. 63–73. DOI 10.31857/S0041377121010053. EDN VYZZGU</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Гринчук</surname> <given-names>Т. М.</given-names> </name>  <name> <surname>Шилина</surname> <given-names>М. А.</given-names> </name>  </person-group>   <article-title>Влияние криоконсервации на стабильность кариотипа трансформированных фибробластов легкого китайского хомячка in vitro</article-title> <source>Цитология</source> <year>2021</year>  <issue>1</issue> <fpage>63</fpage> <lpage>73</lpage> <pub-id pub-id-type="doi">10.31857/S0041377121010053</pub-id> <pub-id pub-id-type="custom" custom-type="edn">VYZZGU</pub-id>       </element-citation> </ref>  <ref id="ref2"> <label>2</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Фибробласты дермы в фокусе современной косметологии: старение и ответ на косметологические процедуры / Л.В. Кирсанова, Е.Р. Аравийская, М.Г. Рыбакова [и др.] // Consilium Medicum. – 2024. – Т. 26. №8. – С. 541–549.</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Кирсанова</surname> <given-names>Л. В.</given-names> </name>  <name> <surname>Аравийская</surname> <given-names>Е. Р.</given-names> </name>  <name> <surname>Рыбакова</surname> <given-names>М. Г.</given-names> </name>  </person-group>   <article-title>Фибробласты дермы в фокусе современной косметологии: старение и ответ на косметологические процедуры</article-title> <source>Consilium Medicum</source> <year>2024</year> <volume>26</volume> <issue>8</issue> <fpage>541</fpage> <lpage>549</lpage>         </element-citation> </ref>  <ref id="ref3"> <label>3</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Влияние длительности хранения криоконсервированных культивированных клеток кожи человека на их жизнеспособность / Н.В. Фирсова, В.А. Савельева, Н.А. Ленгесова [и др.] // Фундаментальные и прикладные исследования по приоритетным направлениям биоэкологии и биотехнологии: сборник материалов VI Всероссийской научно-практической конференции с международным участием / гл. ред. Е.И. Антонова. – Чебоксары: Среда, 2023. – С. 160–167. DOI 10.31483/r-106721. EDN BYMDDL</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Фирсова</surname> <given-names>Н. В.</given-names> </name>  <name> <surname>Савельева</surname> <given-names>В. А.</given-names> </name>  <name> <surname>Ленгесова</surname> <given-names>Н. А.</given-names> </name>  </person-group>   <article-title>Влияние длительности хранения криоконсервированных культивированных клеток кожи человека на их жизнеспособность</article-title>     <fpage>160</fpage> <lpage>167</lpage> <pub-id pub-id-type="doi">10.31483/r-106721</pub-id> <pub-id pub-id-type="custom" custom-type="edn">BYMDDL</pub-id>       </element-citation> </ref>  <ref id="ref4"> <label>4</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Ekpo M.D., Boafo G.F., Xie J. [et al.]. Strategies in developing dimethyl sulfoxide (DMSO)-free cryopreservation protocols for biotherapeutics // Front. Immunol. – 2022. – 13:1030965.</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Ekpo</surname> <given-names>M. D.</given-names> </name>  <name> <surname>Boafo</surname> <given-names>G. F.</given-names> </name>  <name> <surname>Xie</surname> <given-names>J.</given-names> </name>  </person-group>   <article-title>Strategies in developing dimethyl sulfoxide (DMSO)-free cryopreservation protocols for biotherapeutics</article-title>               </element-citation> </ref>  <ref id="ref5"> <label>5</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Gao D. [et al.]. Mitochondrial dysfunction in cryopreserved fibroblasts: Role of antioxidants // Free Radical Biology and Medicine. – 2022. – №180. – P. 1–10.</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Gao</surname> <given-names>D.</given-names> </name>  </person-group>   <article-title>Mitochondrial dysfunction in cryopreserved fibroblasts: Role of antioxidants</article-title> <source>Free Radical Biology and Medicine</source> <year>2022</year>  <issue>180</issue> <fpage>1</fpage> <lpage>10</lpage>         </element-citation> </ref>  <ref id="ref6"> <label>6</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Guan H., Jia C.Y., Chen B. [et al.]. Influence of different thawing temperature on the morphology and type I collagen metabolism of the human fibroblasts processed at – 10 degrees C in vitro // Zhonghua Shao Shang Za Zhi. – 2005. – №5. – P. 370–373.</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Guan</surname> <given-names>H.</given-names> </name>  <name> <surname>Jia</surname> <given-names>C. Y.</given-names> </name>  <name> <surname>Chen</surname> <given-names>B.</given-names> </name>  </person-group>   <article-title>Influence of different thawing temperature on the morphology and type I collagen metabolism of the human fibroblasts processed at</article-title> <source>Zhonghua Shao Shang Za Zhi</source> <year>2005</year>  <issue>5</issue> <fpage>370</fpage> <lpage>373</lpage>         </element-citation> </ref>  <ref id="ref7"> <label>7</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Humphrey S., Brown S.M., Cross S.J. Defining skin quality: clinical relevance, terminology, and assessment // Dermatol Surg. – 2021. – Vol. 47. №7. – P. 974–981. DOI 10.1097/dss.0000000000003079. EDN LXKGLG</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Humphrey</surname> <given-names>S.</given-names> </name>  <name> <surname>Brown</surname> <given-names>S. M.</given-names> </name>  <name> <surname>Cross</surname> <given-names>S. J.</given-names> </name>  </person-group>   <article-title>Defining skin quality: clinical relevance, terminology, and assessment</article-title> <source>Dermatol Surg</source> <year>2021</year> <volume>47</volume> <issue>7</issue> <fpage>974</fpage> <lpage>981</lpage> <pub-id pub-id-type="doi">10.1097/dss.0000000000003079</pub-id> <pub-id pub-id-type="custom" custom-type="edn">LXKGLG</pub-id>       </element-citation> </ref>  <ref id="ref8"> <label>8</label> <citation-alternatives>  <mixed-citation xml:lang="ru">Naing A.H., Kim C.K. A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering // Biotech. – 2019. – №9 (9) : 329.</mixed-citation>    </citation-alternatives> <element-citation publication-type="other">  <person-group person-group-type="author">  <name> <surname>Naing</surname> <given-names>A. H.</given-names> </name>  <name> <surname>Kim</surname> <given-names>C. K.</given-names> </name>  </person-group>   <article-title>A brief review of applications of antifreeze proteins in cryopreservation and metabolic genetic engineering</article-title> <source>Biotech</source> <year>2019</year>  <issue>9 (9)</issue>           </element-citation> </ref>  </ref-list> </back>  </book>