Fundamental and applied research for key propriety areas of bioecology and biotechnology
- Authors:
- Степанкова Дарья Андреевна, Polina I. Elboeva, Ekaterina V. Martynova, Iurii N. Davidiuk, Emmanuel Kabve, Natalia I. Andreeva, Svetlana F. Khaibullina
- Work direction:
- Молекулярная биология
- Abstract:
- Hemorrhagic fever with renal syndrome (HFRS) remains one of the leading natural focal infections in Russia, characterized by pronounced clinical variability. Clinical manifestations of HFRS, in particular, may be determined by single nucleotide polymorphisms (SNPs) in early immune response genes in patients. We determined the allele and genotype frequencies of SNPs in the CASP1 (rs580253) and IL‑6 (rs1800795) genes. CASP1 encodes caspase‑1, an enzyme that is the product of the activated inflammasome, while IL‑6 encodes interleukin‑6, a cytokine of the acute immune response. It was found that the genotype frequencies in the control group conformed to the Hardy–Weinberg law, whereas in HFRS patients a statistically significant deviation from the expected equilibrium was detected, which suggests the existence of correlations between the genotypes of the studied SNPs and the clinical manifestations of the disease.
- Keywords:
- CASP1, IL-6, Hemorrhagic fever with renal syndrome, single nucleotide polymorphisms, NLRP3 inflammasome, allele-specific PCR
Fundamental and applied research for key propriety areas of bioecology and biotechnology